Coding

Part:BBa_K523003:Design

Designed by: Mun Ching Lee, Sylvia Ispasanie   Group: iGEM11_Edinburgh   (2011-07-29)

malS for BioSandwich


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
    Illegal AgeI site found at 1505
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

The part was created from a lab E. coli strain using primers:

  • ACT AGATCT atg aaa ctc gcc gcc tgt ttt c
    • Forward; adds BglII site; starts at start codon.
  • ACT ACTAGT ACC ctg ttg ccc tgc cca gac g
    • Reverse; adds SpeI site; RFC23 and BioSandwich compliant; no stop codons, adds a glycine.


Source

The source E. coli genome sequence can be seen using GenBank U00096.2

References